Cytogenetics - Wikipedia
Nikon Instruments | Nikon Global | Nikon Small WorldThe peroxisome organelles present in the African water mongoose cell culture (APM line) shown above were immunofluorescently labeled with Alexa Fluor 750 conjugated to rabbit secondary antibody fragments directed against rabbit primary antibodies to peroxisomal membrane protein 70 (PMP 70), a major peroxisome membrane polypeptide. In addition, the culture was treated with Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, targeting cytoskeletal F-actin and the mitochondrial network, respectively. Hoechst 33342 was employed as a nuclear counterstain. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, with the exception of Alexa Fluor 750, which was pseudocolored yellow.Share this page:Get updates on our social media channels:Nikon Instruments Inc.© 2017 Nikon Instruments Inc.
Nikon Instruments | Nikon Global | Nikon Small WorldFluorescence emission intensity from a culture of canine kidney epithelial (Madin-Darby;MDCK line) cells stained with the carbocyanine dye DiD, which targets lipophilic organelles, including the plasma membrane. The visible light absorption maximum of DiD is 644 nanometers and the emission maximum occurs at 665 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Carbocyanine probes are often utilized in apoptosis experiments and as tracers for cellular migration.Share this page:Get updates on our social media channels:Nikon Instruments Inc.© 2017 Nikon Instruments Inc.